The C4D Test

C4D test is an innovative test that can measure the quantity of Non-Cp copper with serum, through a simple blood sampling. The test is accurate, sensitive and reliable to measure Non-Cp copper circulating through a fluorescent probe set up ad hoc (Ref Patent: PCT/EP2012/072063)

For whom is the test intended?

Lapses of memory during the day are something very common because of the stress imposed by the modern rhythms of life and sometimes they are associated with the aging process. Nevertheless, when these lapses of memory seem to condition the daily activities, such as the simplest functions like eating, washing, walking, etc., you should talk to the family doctor about these problems or ask him/her further information, for example the Alzheimer Value Unit (UVA). The UVA are specialized centers of the National Health System headed by geriatricians, neurologists or psychologists who can identify and evaluate the seriousness of these lapses; they can ask for in-depth specific exams of the cognitive disturbances and if necessary, they can call for a series of interventions for the patient and his/her family, for free medicines and the appropriate services. If you want to know what are the 10 predominant symptoms of Alzheimer’s disease, you can consult the guidelines of the American Academy of Neurology at

Besides all the exams that can evaluate this cognitive disturbance (, ntoday the C4D Test is available: it is a simple and reliable test which identifies an altered copper metabolism which could be a contributory cause or a risk factor of the progression of the disease. Correcting this could be achieved by changing “life style”, i.e. diet, food and excercise.

How to test?

A simple blood test sampling at authorized analysis laboratories or at an affiliated UVA is sufficient.

How to interpret the C4D test? 

The test supplies a quantitative value of Non-Cp copper concentration. If this value is in the range of normal population values, the test indicates that the patient is not affected by copper dysfunction but he or she must consult a doctor in order to interpret the results.

If the value is not within the normal ramge, the C4D test is positive and the patient must consult a specialized doctor to affront these problems and reactivate a normal metabolism: for example using a balanced, low copper diet or taking Zinc supplements which are able to increase the production of endogenous proteins of copper storage in the body. The metallothionein, which captures the circulating Non-Cp copper, favors its excretion through the faeces, reducing its quantity in the blood and its toxicity.

How to measure?

The test works in a very simple way: it is a fluorescent probe. The tool is composed of a part which emanates a fluorescent signal which later, is quantified by a fluorimeter and a portion which aims at catching the Non-Cp copper in the serum. When this metal is captured, the probe portion modifies itself changing the fluorescent portion and turns off the signal.

A fluorimeter reads the emission change which is proportional to the quantity of the Non-Cp present in the blood sample.